FIG 5.

Limited translocation of the SPI-2 effector PipB2 in nongrowing intracellular S. Typhimurium. (A) Distribution of endogenous chromosomally tagged PipB2-HA in NRK-49F fibroblasts at 24 h postinfection. (B) Mechanical fractionation denotes that endogenous PipB2-HA is not released to the endomembrane system. P1, nuclear/bacterial fraction; P2, high-speed particulate material, membranous fraction; S2, cytosol, soluble fraction (see Materials and Methods for details). (C) PipB2 is translocated by dormant intracellular bacteria, as denoted by its detection in a subcellular fraction containing material solubilized with 1% (vol/vol) Triton X-100. (D) Distribution of endogenous PipB2-HA in HeLa epithelial cells at 6 h postinfection. Arrows indicate the presence of the effector in endomembranous filamentous structures.