FIG 2.

Increased host cell death was detected in the wound tissue from diabetic mice on insulin therapy. Tissue was harvested from the wound margin on the fourth day of infection from nondiabetic (ND), diabetic (D), and diabetic mice treated with insulin (D+I). (A) TUNEL and caspase-3 immunofluorescent staining was performed on sections of formalin-fixed wound tissue. Images were taken at the same exposure (200 ms), and fluorescent intensity, corresponding to TUNEL and caspase-3, was measured in two sections from 3 mice per group. Blue color, DAPI-stained nuclei; green, TUNEL or caspase-3. (B) ImageJ analysis of green fluorescent intensity indicative of TUNEL and caspase-3 staining. One-way ANOVA, followed by a Tukey-Kramer multiple-comparison test, was used to determine the differences between groups. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (n = 6 images).