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The role of RcsB-regulated genes in serum survival. (A) CFT073 with deletions in exopolysaccharide (wca and yjbE) and lysozyme (ydhA) production genes were adjusted to ∼3 × 108 CFU/ml in LB. (B) CFT073 and deletion mutants (CFT073 ΔrcsB* and CFT073 Δwca*) carrying either empty or recombinant vector were adjusted to ∼3 × 108 CFU/ml in LB. Bacterial cells were incubated with 50% NHS or HIS at 37°C. Viability of bacteria is plotted in CFU/ml and tested by the paired Student t test.
