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. 2014 Jan;82(1):445–452. doi: 10.1128/IAI.01238-13

FIG 1.

FIG 1

Construction of the plzA mutant and complemented strains. (A) Schematic representation of the genes bb0732 to bb0735 in the Borrelia chromosome and replacement of plzA (bb0733) with an aadA gene cassette (conferring streptomycin resistance) by homologous recombination. A shuttle vector carrying a copy of a constitutive flgB promoter-driven plzA gene encoding a FLAG-tagged PlzA was used for complementation of the plzA mutant (plzA/flgBp-plzA). (B) Confirmation of the plzA mutation and complementation by RT-PCR. flaB serves as a positive control. Lane 1, wild-type B. burgdorferi strain B31-5A4NP1; lane 2, the plzA mutant; lane 3, the plzA mutant carrying flgBp-plzA. (C) qRT-PCR analysis of plzA levels in the plzA mutant and the plzA mutant carrying flgBp-plzA.