FIG 2.

PlzA controls production of RpoS and OspC. Wild-type B. burgdorferi B31-5A4NP1 (WT), the plzA mutant (plzA), and the plzA mutant carrying a constitutive copy of plzA (plzA/flgBp-plzA) were harvested at late logarithmic phase, and the whole-cell lysates were subjected to SDS-PAGE (Coomassie blue stain) (top) and immunoblotting (bottom, using the chemiluminescent method). For immunoblotting, a mix of monoclonal antibodies against FlaB, RpoS, and OspC were used as primary antibodies, and the image was developed by chemiluminescence. FlaB served as a loading control.