FIG 5.

Transcriptional and posttranscriptional control of bosR expression by PlzA. (A) Wild-type B. burgdorferi B31-5A4NP1 (WT), the rrp1 mutant (rrp1), the rrp1 mutant with a wild-type copy of rrp1 (rrp1^com), the rrp1 mutant carrying a constitutive copy of plzA (rrp1/flgBp-plzA), and the plzA mutant (plzA) were harvested at stationary phase, and the whole-cell lysates were subjected to immunoblot analyses using the chemiluminescent method. (B) RNAs were extracted from the cells described above and subjected to qRT-PCR analysis for bosR transcript levels.