TABLE 1.
Primers used in this study
| Primer name | Sequence (5′–3′) | Purpose |
|---|---|---|
| bb0733-AF | CAGGTACCAATTCCAAGTATAGCTCCAAAACTTG | Construct pMH23 |
| bb0733-AR | CAGGATCCGAATCCATAGAAATAGAATAAATTACTC C | Construct pMH23 |
| bb0733-BF | CACTGCAGATTTTTAGTTTTGAATTTATAGATGGAG | Construct pMH23 |
| bb0733-BR | CACTCGAGCTCTTAAGATGGCATCAATTAAATTTC | Construct pMH23 |
| KPNflgBp-5 | CAGGTACCATGTTTAAGGTTTATGATTTAG | Construct pMH105 |
| flgBp-733-3 | CCATAATCTCTTATTTTTCTAGATAAAAGCATATGGAAACCTCCCTCATTTAAAATTGC | Construct pMH105 |
| flgBp-733-5 | GCAATTTTAAATGAGGGAGGTTTCCATATGCTTTTATCTAGAAAAATAAGAGATTATGG | Construct pMH105 |
| Pst-Flag-733-3 | CACTGCAGTTACTTGTCGTCATCGTCTTTGTAGTCATTGAAATAATCATGGATCAAC | Construct pMH105 |
| qBB0680F | TTGAGCAAATAGCCTCAGGT | qRT-PCR |
| qBB0680R | TCCACAATGTCTTGCATAGC | qRT-PCR |
| qBB0566F | GCCTGAAGGAGAGCTTGTAA | qRT-PCR |
| qBB0566R | ATCCCCTTCTTTCATTTTCTT | qRT-PCR |
| qBBA73F | AAACAACACAGAAGCGATAA | qRT-PCR |
| qBBA73R | AGATTTTGGGTGCTTACAAT | qRT-PCR |
| qBBA24F | GATACCCCACTACCCGTTTT | qRT-PCR |
| qBBA24R | CATGTGGACTAACAGGAGCA | qRT-PCR |
| qBBA07F | ACGAAGCAGATGCATCATAA | qRT-PCR |
| qBBA07R | AATGTTGCCAATGCTAAACA | qRT-PCR |
| qBB0771F | TAACCCTTTACCCGCATATC | qRT-PCR |
| qBB0771R | TACAAAGAGGCAATGCAAAA | qRT-PCR |
| qBB0733F | ATGCTTTTATCTAGAAAAATAAGAGATTATGG | qRT-PCR |
| qBB0733R | ATTGAAATAATCATGGATCAACATAG | qRT-PCR |
| qBB0844F | CATGCACCTCTGCTTGTATT | qRT-PCR and RT-PCR |
| qBB0844R | ATTAGCGATGGGAGTCTTGA | qRT-PCR and RT-PCR |
| qPCR-flaB-R | CAGCAATAGCTTCATCTTGGTTTG | qRT-PCR and RT-PCR |
| qPCR-flaB-F | ACCAGCATCACTTTCAGGGTCTCA | qRT-PCR and RT-PCR |
| qBB0733F | ATGCTTTTATCTAGAAAAATAAGAGATTATGG | qRT-PCR |
| qBB0733R | ATTGAAATAATCATGGATCAACATAG | qRT-PCR |
| qBBB19F | CGGATTCTAATGCGGTTTTACTTG | qRT-PCR |
| qBBB19R | CAATAGCTTTAGCAGCAATTTCATCT | qRT-PCR |