FIG 3.

Macrophage killing of intracellular yeasts and ROS production. (A) Infected macrophages were lysed after 24 h, and WT, KO, and REC yeast mortality was assessed. (B) Fluorimetric measurement of intracellular ROS production by macrophages infected with the WT, the WT plus 10 μM tyrosol (Tyr), the KO, the KO plus 10 μM PEA, or the REC strain. Noninfected macrophages produced basal levels of ROS production that were designated “0.” Results are expressed as mean values ± SD of data from three independent experiments. Means with different letters are significantly different (P < 0.05). A.F.U., arbitrary fluorescence units.