FIG 1.

Expression of ankB upon intimate attachment to hMDMs. Quantitative real-time PCR of ankB gene expression by L. pneumophila at 0, 7.5, and 15 min after attachment to hMDMs. The hMDMs were pretreated with cytochalasin D and infected for the indicated time periods, followed by total isolation of RNA and RT-PCR. Expression of ankB or the control gene mompS was compared to 16S RNA levels. Fold changes in gene expression were compared to levels measured at time zero min. Error bars indicate standard errors of the means (SEM). The data are representative of three independent experiments.