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Translocation of BlaM-AnkB fusion protein by L. pneumophila upon intimate attachment to U937 cells. Cytochalasin D-treated U937 cells were infected with increasing MOIs of L. pneumophila strains expressing various BlaM fusion constructs, and translocation was measured by monitoring hydrolysis of the fluorogenic substrate CCF4. A 460 nm/530 nm ratio greater than 1 indicates translocation of the fusion construct. Data represent mean values of three independent experiments.
