FIG 3.

Anchoring of native AnkB injected by attached L. pneumophila to the cytosolic side of the plasma membrane of hMDMs beneath bacterial attachment sites. Representative confocal microscopy images of cytochalasin D-treated infected hMDMs that were preloaded with anti-AnkB antisera (red) in the absence (A) or presence (B) of digitonin. The hMDMs were infected by L. pneumophila strains (green) for 15 min. The numbers in the merged images of all panels are means and standard deviations of the frequency of anchoring AnkB to the plasma membrane beneath attached extracellular bacteria. The data represent analyses of 100 infected cells and are representative of three independent experiments.