FIG 4.

The farnesylation machinery components FTα, RCE1, and IcmT are recruited beneath attachment sites of L. pneumophila to hMDMs. The hMDMs pretreated with cytochalasin D were infected by wild-type (WT) L. pneumophila and the isogenic dotA or ankB mutant for 15 min. Representative confocal microscopy images of infected hMDMs showing colocalization of FTα (A), RCE1 (B), and IcmT (C) proteins to attached WT, ankB mutant, or dotA mutant bacteria. Bacteria were labeled with anti-Lpn antibody (green), and FTα, RCE1, and IcmT were labeled with the respective specific antibodies (red) and then analyzed by confocal microscopy. The arrowheads indicate intense colocalization of FTα, RCE1, or IcmT with the WT strain. The numbers in the merged images of all panels are means and standard deviations of the frequency of recruitment of FTα, RCE1, or IcmT beneath attached extracellular bacteria. The data represent analyses of 100 infected cells and are representative of three independent experiments.