FIG 5.

Invasion-inhibitory activity of anti-PfRH5 rabbit antibodies. (A) Invasion-inhibitory activity of purified rabbit total IgG (0.5 to 10 mg/ml) against rPfRH5₆₃ and the receptor binding domains of other key merozoite ligands (PfRH1, PfRH2, PfRH4, PfAARP, and PfF2) against the P. falciparum clone 3D7. AMA-1 IgG (5 mg/ml) was used as a positive control. (B) Strain-transcending parasite neutralization activity of anti-PfRH5 total rabbit IgG (0.5 to 10 mg/ml) against five diverse P. falciparum clones. The control anti-HPR total IgG failed to exhibit any invasion inhibition at the maximum concentration of 10 mg/ml. The results represent the averages of three independent experiments performed in duplicate. The error bars represent the standard errors of the means.