FIG 1.

Translocation of the 65-kDa RelA (p65) NF-κB subunit to the cell nucleus. (A) Western blot of cytoplasmic (lanes 1, 3, and 5) and nuclear (lanes 2, 4, and 6) fractions (10 μg/lane) of M. bovis-infected monocytes pooled from 3 animals. Lanes: M, molecular mass marker; 1 and 2, untreated monocytes; 3 and 4, M. bovis-infected monocytes; 5 and 6, LPS-treated monocytes. (B) To confirm successful separation of nuclear and cytoplasmic fractions, the nuclear histones (15 kDa) are shown in the nuclear fractions (lanes 2, 4, and 6). The experiment was performed 3 times, and this figure is representative.