FIG 2.
Consumption of O2 by fur mutant Salmonella undergoing nitrosative stress. The consumption of O2 was recorded in Salmonella grown to an OD600 of 0.5 in EG medium. The bacteria were diluted to an OD600 of 0.2 in an air-sealed chamber. Bacterial cultures were treated with 5 μM proli NONOate (arrows) at a time when the concentration of O2 in the medium was about 130 μM. The evolution of NO in the cultures was also recorded. The data are representative of those from three independent experiments performed on three separate days.