TABLE 1.
Oligonucleotides used in this study
| Oligonucleotide | Sequencea (5′–3′) | Use in this work |
|---|---|---|
| Crasp-1-Bam | GCTAAAACTTCTCTTTTTTTTAGGATCCCAACCCAAATCC | Cloning of cspA LW2 in pKFSS1 |
| Crasp-1-Hind | GAAAGAAAAAAAATAAGCTTTTGCACTTGATATTTTTAAAAAG | Cloning of cspA LW2 in pKFSS1 |
| BaCRASP-1(+)Bam | GCCAGTGTGCTGGGATCCGCCCTTCATTGC | Cloning of cspA PKo and cspA A14S in pKFSS1 |
| BaCRASP-1(−)Hind | GATGGATATCTGCAAGCTTCGCCCTTGGT | Cloning of cspA PKo and cspA A14S in pKFSS1 and cloning of cspA PKo in pQE-30Xa |
| BBA68 Bam | ACCGGATCCGCACCTTTTAGC | Cloning of cspA LW2 in pQE-30 Xa |
| BBA68 Eco 2 | TTTCGAATTCTTAGTAAAAGGCAGGTTTTAAAG | Cloning of cspA LW2 in pQE-30 Xa |
| BsCRASP-1 Bam | CTTTAATTTGCATCGGATCCACGCCTATTAATAAC | Cloning of cspA PKo in pQE-30 Xa |
| pGEX(+) | GGGCTGGCAAGCCACGTTTGGTG | Recloning of cspA A14S in pQE-30 Xab |
| pGEX-CSPA Pst(−) | CCGAAACGCGCGCTGCAGATCGTCAGTCAGTCACG | Recloning of cspA A14S in pQE-30 Xab |
| aadA+NdeI | CATATGAGGGAAGCGGTGATC | Amplification of aadA |
| aadR+AatII | GACGTCATTATTTGCCGACTACC | Amplification of aadA |
| Fla6 | AACACACCAGCATCGCTTTCAGGGTCT | Amplification of flaB |
| Fla7 | TATAGATTCAAGTCTATTTTGGAAAGCACCTA | Amplification of flaB |
a
Sequences of specific restriction endonuclease recognition sites are underlined.
b
Plasmid pGEXcspA14 (56) was used as the template for recloning of the cspA gene of B. spielmanii A14S into pQE-30 Xa.