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. Author manuscript; available in PMC: 2014 Feb 3.
Published in final edited form as: Nat Med. 2013 Mar 24;19(4):473–480. doi: 10.1038/nm.3117

Figure 5. Down-regulation of C/EBPβ via over-expression of miR-155 results in decreased SNX27 levels in Down syndrome brains.

Figure 5

(a) Western blot analysis of SNX27, C/EBPβ, APP and GluR1 in the cortex of age-matched controls (Normal) and Down syndrome individuals (DS). Signal intensity of the immunoblots was calculated and normalized to β-actin; n = 7. P values were calculated using a nonparametric t test, *P < 0.05.

(b) Western blot analysis of SNX27 expression in the cortex of human with sporadic Alzheimer’s disease and control brains. Signal intensity of the immunoblots was normalized to β-actin; n = 8. P values were calculated using a nonparametric t test, *P < 0.05.

(c) Quantitative RT-PCR analyses of SNX27 mRNA levels in the cortex of Down syndrome individuals and normal controls. Data represent mean ± s.e.m., n = 7. P values were calculated using a nonparametric t test, *P < 0.05.

(d) Western blot analyses of C/EBPβ and SNX27 protein levels in MC-IXC cells transfected with miR-155 or control mimic. Data represent mean ± s.e.m., n = 3. P values were calculated using two-tailed Student’s t test, **P < 0.01.

(e) Quantitative RT-PCR analyses of SNX27 and C/EBPβ mRNA levels in MC-IXC cells transfected with miR-155 or control mimic. Data represent mean ± s.e.m., n = 3. P values were calculated using two-tailed Student’s t test, *P < 0.05, **P < 0.01.

(f) Western blot analysis for SNX27 expression in MC-IXC cells transfected with LAP2 or control. Data represent mean ± s.e.m., n = 3. P values were calculated using two-tailed Student’s t test, *P < 0.05.

(g) Western blot analysis of Snx27 expression in the cortex and hippocampus from Cebpb+/− and Cebpb−/− mice. Data represent mean ± s.e.m., n = 3. P values were calculated using two-tailed Student’s t test, *P < 0.05.

(h) Firefly luciferase reporter assay for SNX27 promoter activity. Data represent mean ± s.e.m., n = 4. P values were calculated using two-tailed Student’s t test, *P < 0.05.

(i) Binding of C/EBPβ to the SNX27 promoter as determined by a Chromatin IP assay.