Figure 3. sRNAs identified by different studies [2]–[4] and this study and their overlap. sRNAs for this study were identified via automatic identification with our newly developed pipeline.

144 (55%) known sRNAs were recovered with the automated method. Of the 711 sRNAs identified in total, 569 were yet undescribed. The majority of these, however, were later removed due to their likely origin as transcription start site and 5′ UTR of known genes. Most of sRNAs, which were not recalled by the automated method, were found by manual reevaluation, increasing the total recall rate to 90%.