Figure 3. Culture fluids of lineage⁻CD34⁺CD31⁺ cells are inhibitory on HBD-1 production by NHEK. A.

Inhibition of HBD production in NHEK cultures supplemented with culture fluids of lineage⁻CD34⁺CD31⁺ cells. Culture fluids were harvested 48 hours after cultivation of lineage⁻CD34⁺CD31⁺ cells (1×10⁶ cells/ml) derived from burn patients #8, #9, #13, #14 and #15 (open circles). The culture fluids of lineage⁻CD34⁺CD31⁻ cells of healthy donors #1∼#5 (filled circles) were utilized as a control. Five to 40% (v/v) of these culture fluids were added to cultures of NHEK (1×10⁵ cells/ml). Culture fluids harvested from NHEK cultures were assayed for HBD-1 by ELISA. * P<0.01 vs control. B–D. Production of IL-10, IL-13 and CCL2 by lineage⁻CD34⁺CD31⁺ cells. Lineage⁻CD34⁺CD31⁺ cells (1×10⁵ cells/ml, open circles) isolated from peripheral blood of burn patients #8, #9, #13, #14 and #15 were cultured for 12 to 48 hours. As controls, peripheral blood lineage⁻CD34⁺CD31⁻ cells of healthy donors #1∼#5 (filled circles) were cultured under the same conditions. Supernatants obtained were assayed for IL-10 (B), IL-13 (C) and CCL2 (D) by ELISA. * P<0.05, ** P<0.01 vs lineage⁻CD34⁺CD31⁻ cells.