Figure 3. Time courses of phenotypes in strains depleted for Rat1.

(A) OD₆₀₀ of the cultures at the time points indicated. Non-depleted cells, growing in the absence of methionine, were maintained in exponential growth phase by frequent dilution with pre-warmed medium. Rat1-depleted cells, growing in the presence of methionine, were diluted at the same times and to the same extent as non-depleted cells. OD₆₀₀ values (cell density) for the non-depleted strain at the time points indicated are shown in orange. OD₆₀₀ values for the Rat1-depleted strain at the same time points are indicated in purple. Doubling time for the non-depleted strain was ∼100 min. (B) Relative abundances of 5.8S(L) (Rat1-independent) and 5.8S(S) (Rat1-dependent), with total 5.8S rRNA abundance set to 100% at each time point. Graphs show the averages of three independent experiments.