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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Oncogene. 2012 Nov 5;32(40):4814–4824. doi: 10.1038/onc.2012.494

Figure 2. Mitochondria are more fragmented in metastatic breast cancer cells.

Figure 2

(a) Comparison of migration and invasion abilities of breast cancer MCF7, MDA-MB-231 and MDA-MB-436 cells. n=5, mean ± s.e.m., *p<0.01. (b) Representative images of MCF7, MDA-MB-231 and MDA-MB-436 cells, stained with MitoTracker Red, show mitochondrial morphology (left panel), analyzed by measuring mitochondrial length (right panel). Scale bars, 5 μm. (c) Western blot analysis of Drp1, pS616-Drp1, Mfn1 and Mfn2 expression levels in MCF7, MDA-MB-231 and MDA-MB-436 cells using anti-Drp1, -pS616-Drp1, -Mfn1 and -Mfn2 antibodies (left panel), analyzed by measuring band density (right panel). β-Actin was used as an internal control. n=3, mean ± s.e.m., *p<0.01 as compared to that of MCF7 cells.