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. 2014 Feb 3;9(2):e87302. doi: 10.1371/journal.pone.0087302

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© 2014 Antoine et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Zebrafish encoding 3-OST-4 plasmid was constructed by inserting the open reading frame of 3-OST-4 into pcDNA3.1 and the construct was designated pcDNA3.1-ZF-3-OST-4. The inserted sequence of 1278 bp of 3-OST-4 was verified after digestion using BamH1 and Xho1. (C–D). RT-PCR analysis for ZF encoded 3-OST-2 and 3-OST-4 isoforms expression in CHO-K1 cells. The housekeeping gene GAPDH was used as a normalization control.