Figure 6. TbPRMT6 does not methylate T brucei histone tails.

A–E: Two micrograms of histone tail peptides were incubated with 3 µg of TbPRMT6 in the presence of SAM and then subjected to MALDI-TOF MS analysis F: Comparison of N terminal tails of yeast, T brucei, and human histones The arginine residues conserved among all the three species are shown in red, and the arginine residues that are conserved in yeast and human but not conserved in T brucei are colored in green Those arginine residues that subjected to methylation in yeast or mammal are labeled with red balls.