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. 2014 Feb 3;9(2):e87537. doi: 10.1371/journal.pone.0087537

Figure 5. Kaiso binds the β-catenin promoter region via methylated CpG dinucleotide sequences.

Figure 5

No specific bands appear in the KBS binding region (A), while a specific band appears in the methylated CpG dinucleotide sequence region in SPC cell line (B). C: Luciferase reporter vectors and pRL-TK Vector were co-transfected into SPC cells with either the control vector or kaiso expressing plasmid DNA. They were then compared with cells treated with demethylating agents to assess the importance of the Kaiso binding domain. Statistical analysis by ANOVA indicated that the relative luciferase activity in the cell group with methylation site reporter vectors and Kaiso plasmid were higher than the other cell groups (P = 0.000, F = 83.018). No apparent changes in activity were observed in the SPC cells that were treated with demethylating agents (P = 0.374, F = 1.187). D: Luciferase activity obtained from the mutant construct showed no difference in any of these conditions (P = 0.674, F = 0.641). Similar results of ChIP (E, F) and luciferase analyses (G: P = 0.000, F = 37.703; P = 0.569, F = 0.718; H: P = 0.762, F = 0.513, respectively) were observed in the LTE cell line.