Table 1. β-catenin promoter-specific primers for bisulfite (BSP) sequencing.
Name | Sequence (5′-3′) | Length | Tm | size |
1 F | TGCGATTTAGGTTTAGTAGGGAGTGT | 26 | 62.4 | 345 |
1 R | AATATCCTCCCCTATCCCAAACC | 24 | 62 | |
2 F | ATAGGGGAGGATATTAGGGTTATT | 24 | 56.7 | 336 |
2 R | TAACGCCGCACAAAAAACTCTTAT | 24 | 62.7 | |
3-1 F | GGAGGAAGGTTTGAGGAGTAGTTTTAG | 27 | 62.3 | 387 |
3-1 R | CCGCCTACCATCCG/AACTCCTATA | 24 | 65.7 | |
3-2 F | TATAGGAGTTCGGATGGTAGG | 21 | 54 | 232 |
3-2 R | CCCCAAAACTAATAAAACTTAAAATAAC | 28 | 58.8 | |
3-3F | CGGCGTTATTTTAAGTTTTTCG | 22 | 59.5 | 104 |
3-3 R | AAACTACTCCTCAAACCTTCCT | 22 | 53.9 | |
4F | GTTGAAAAATTAAGATATGGGTTAG | 25 | 54.7 | 355 |
4R | CTATAAACCTAAACTAATATATTCATATC | 29 | 51.4 |