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. 2014 Feb 3;9(2):e87537. doi: 10.1371/journal.pone.0087537

Table 1. β-catenin promoter-specific primers for bisulfite (BSP) sequencing.

Name Sequence (5′-3′) Length Tm size
1 F TGCGATTTAGGTTTAGTAGGGAGTGT 26 62.4 345
1 R AATATCCTCCCCTATCCCAAACC 24 62
2 F ATAGGGGAGGATATTAGGGTTATT 24 56.7 336
2 R TAACGCCGCACAAAAAACTCTTAT 24 62.7
3-1 F GGAGGAAGGTTTGAGGAGTAGTTTTAG 27 62.3 387
3-1 R CCGCCTACCATCCG/AACTCCTATA 24 65.7
3-2 F TATAGGAGTTCGGATGGTAGG 21 54 232
3-2 R CCCCAAAACTAATAAAACTTAAAATAAC 28 58.8
3-3F CGGCGTTATTTTAAGTTTTTCG 22 59.5 104
3-3 R AAACTACTCCTCAAACCTTCCT 22 53.9
4F GTTGAAAAATTAAGATATGGGTTAG 25 54.7 355
4R CTATAAACCTAAACTAATATATTCATATC 29 51.4