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. 2014 Feb 3;9(2):e87417. doi: 10.1371/journal.pone.0087417

Figure 5. The optimal number of reference genes required for effective normalization across different tissues of D. latiflorus Munro.

Figure 5

(A) Different tissues, (B) Different samples from seedlings and anther-regenerated plants of different ploidy, (C) All samples. The pairwise variation (Vn/Vn+1) was analyzed between normalization factors NFn and NFn+1 by geNorm program to determine the optimal number of reference genes.