Table 2. Selected candidate reference genes and target genes, primers and different parameters derived from qRT-PCR analysis.
| Gene Symbol | Primer pair (forward/reverse) | Product Size (bp) | R2 * | PCR efficiency |
| ACT | TGCTCTCCCCCATGCTATCCTTC/ATGTCCCTCACAATTTCCCGCTC | 130 | 0.9991 | 100.09% |
| GAPDH | CCGTCAACGACCCCTTCATCACC/TACTCAGCACCAGCCTCAGCCCA | 193 | 0.9992 | 99.38% |
| NAC | TGATGCTGTGGCTCCTAAGGTTGAA/TAGCAAAAGCAAGAGCTGCCTGATC | 173 | 0.9991 | 100.25% |
| TUA | GTTGGTGGTGGAACTGGGTCAGG/TCAAGCAAGGAGTGGGTGGAGAG | 167 | 0.9980 | 96.74% |
| UBC | CCCAGCCCTTACCTTGAAGACGG/TAACGAGCAGTGGCAACAAATGTGG | 135 | 0.9980 | 97.61% |
| EF1α | GTTGTTACCTTTGGTCCCAGCGG/TGGCAGGGTCATCCTTGGAGTTG | 175 | 0.9984 | 98.55% |
| CYP | GGTGGTGAACCCAAAAATGCTGCTAC/ACCCTTTCTTCCCATTCTCATTATCTGC | 172 | 0.9986 | 99.76% |
| RPL3 | AGGAGTAGGCAGGGGAAGATGTCGC/TTGTAGCCAAGGAAGGCAGTGAGGTG | 167 | 0.9976 | 96.93% |
| 18S | ATGCTTGGCAAATGCTTTCGCTGTA/ATGATTAAGAGGGACTGACGGGGGC | 101 | 0.9984 | 99.48% |
| TEF | CTCTTCGCCACGCCAACCACTTC/GTCGTCCACCGTCCTTGCCACC | 120 | 0.9978 | 98.82% |
| TIP41 | TTGGTTTCTGCTCTTGCGTTTTTGG/ACGGGTTTTGCTTCCTCATTATTTCC | 106 | 0.9991 | 97.69% |
| NTB | TTGCTGTGCTCCAACCAAGATGA/TCAAACAAGACGAGAGCCTGCCT | 153 | 0.9979 | 98.32% |
| Ferritin | TACTTTCGGCTGCGCCATGT/CGGGATGCTGAAGGAAAT | 188 | 0.9980 | 98.66% |
| Laccase | TGGAAGGTGACGTTGTTGTT/TCAATGTGACAATGGCGACT | 153 | 0.9989 | 97.47% |
*
R2 represents the correlation coefficients.