Table 4. Expression stability values of the candidate reference genes calculated using BestKeeper.
Rank | Tissues | Seedlings and different ploidy | Total | |||
Gene name | Coeff. of corr (r)* | Gene name | Coeff. of corr (r)* | Gene name | Coeff. of corr (r)* | |
1 | EF1α | 0.95 | RPL3 | 0.97 | EF1α | 0.92 |
2 | GAPDH | 0.93 | TEF | 0.94 | GAPDH | 0.90 |
3 | RPL3 | 0.92 | EF1α | 0.93 | RPL3 | 0.85 |
4 | TEF | 0.90 | GAPDH | 0.82 | ACT | 0.83 |
5 | ACT | 0.86 | UBC | 0.57 | TEF | 0.76 |
6 | TIP41 | 0.86 | NAC | 0.52 | TIP41 | 0.76 |
7 | NTB | 0.82 | 18S | 0.48 | TUA | 0.68 |
8 | TUA | 0.67 | ACT | 0.35 | NAC | 0.54 |
9 | 18S | 0.51 | TIP41 | 0.28 | UBC | 0.31 |
10 | UBC | 0.46 | NTB | 0.27 | 18S | 0.12 |
Coeff. of corr (r) is the shortage of Coefficient of correlation.