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. 2014 Feb 3;9(2):e87306. doi: 10.1371/journal.pone.0087306

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© 2014 Abu Irqeba et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole cell extracts were prepared from wild type, hog1Δmutants, and hog1^K52R mutants treated with 1 M sorbitol for the indicated time, resolved by 8% SDS-PAGE, and probed with anti-SUMO (top panel) or anti-phospho-Hog1 (middle panel) antibodies. The equal loading of each lane was confirmed by Ponceau S staining (bottom panel). IB, immunoblotting.