Figure 6. Overexpression of SIZ1 enhances osmo-sensitivity of hog1Δ mutants.
a, Osmo-sensitivity of wild type and siz1Δ mutants was measured by comparing the growth of serial diluted exponentially growing yeast cells on YPD plates with or without the addition of 1 M sorbitol. b, wild type (WT) and hog1Δ mutants were transformed with either URA3-marked plasmids that overexpress SIZ1 (i.e., HC-SIZ1) or URA3-marked empty vector. The osmo-sensitivity of the cells was measured by comparing the growth of serial diluted exponential cells on SCG-URA plates with or without the addition of various concentrations of sorbitol (0.5 M and 1 M, respectively).