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. 2014 Feb 3;9(2):e87306. doi: 10.1371/journal.pone.0087306

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© 2014 Abu Irqeba et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Whole cell extracts were prepared from wild type (JD-52) and JD-52 derived mutants either accumulating poly-sumoylated species (ulp2Δ) or incapable of forming poly-sumoylated species (smt3^K11,15,19R and ulp2Δ/smt3^K11,15,19R) treated with 1 M sorbitol for the indicated time, resolved by 8% SDS-PAGE, and probed with anti-SUMO antibodies. IB, immunoblotting. Note that an efficient detection of the very high molecular weight species accumulated in the ulp2Δ mutants requires the antibody (anti-SUMO) to be very fresh.