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. 2014 Feb 3;9(2):e87733. doi: 10.1371/journal.pone.0087733

Figure 4. SIRT1 deficiency induces hyperactivation of NF-κB in BMMs.

Figure 4

BMMs were cultured from mSIRT1 or WT mice and stimulated with TNF-α (10 ng/ml) for 1 h. (A) Immunostaining for acetylated p65 showed that loss of SIRT1 resulted in higher and sustained levels of acetylated p65 in the nuclei of macrophages following TNF-α treatment. (B) The levels of acetylated p65 were analyzed by Western blotting. Representative blot shown is from one of the three independent experiments with similar results. Values  =  mean ± SE, *p<0.05 vs. WT. (C) The DNA binding activity of NF-κB was analyzed by EMSA.