Table 1. Mitochondrial proteins binding to GCDH.

Protein	Gene	Localization	Function
aldehyde dehydrogenase 2	ALDH2	M	oxidation of aldehydes to generate carboxylic acids
dihydrolipoamide-succinyltransferase	DLST	M	component of the 2-oxoglutarate dehydrogenase complex which catalyzes the conversion of 2-oxoglutarate to succinyl-CoA in the tricarboxylic acid cycle and the conversion of α-ketoadipate to glutaryl-CoA in the degradation pathway of lysine
electron transfer flavoprotein subunit beta	ETFB	M	ETF subunit acting as electron acceptor for several acyl-CoA dehydrogenases and transfer the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase
glutamate dehydrogenase 1	GLUD1	M	key enzyme in the nitrogen and glutamate/α-ketoglutarate metabolism
thioredoxin-dependent peroxide reductase	PRDX3	M	member of the peroxiredoxin family of antioxidant enzymes involved in cellular redox regulation
ATP synthase subunit alpha	ATP5A1	IM	part of the F0 domain of ATP synthase, functions as a proton channel
ATP synthase subunit beta	ATP5B	IM	part of the F0 domain of ATP synthase, functions as a proton channel

GCDH-His₆ was immobilized on beads and incubated with isolated mitochondrial matrix proteins from pig liver. The identity of specifically co-purifying proteins was determined by LC-MS/MS.

M: mitochondrial matrix; IM: inner mitochondrial membrane.