Figure 2. Localisation of PfRab5B-GFP fusion proteins in P. falciparum.

Parasites were transfected with constructs expressing the N-terminal 28 amino acids of PfRab5B or 29 amino acids of PfGAP45 fused to GFP under a schizont stage-specific promoter (msp3 5′UTR region). Localisation of PfRab5B₂₈-GFP (A) and GAP45₂₉-GFP fusions (B) as well as myristoylation (G2A) and palmitoylation-deficient fusions (C3A in PfRab5B₂₈-GFP and C5A in GAP45₂₉-GFP) were investigated. The first image in a series corresponds to GFP fluorescence, the second a merge of GFP fluorescence with nuclear DAPI stain, and the third a merge of GFP fluorescence, DAPI and bright field images. Size bars are 2 µm.