Figure 2. Regulation of migration, invasion by Trop-2 in lung cancer cells. (A) Motility assay. Photomicrographs demonstrating the results of the in vitro motility of in lung cancer cells using the simple scratch technique. Lung cancer cells were grown in monolayer, scratched and monitored every 6 h for 24 h. (B) Transwell migration assay. Lung cancer cells were plated in the top chamber of the transwell, cells migrated to the lower chambered were fixed with methanol, stained with crystal violet and counted. Data represent mean ± SD. *Significantly different from respective controls, P < 0.05. (C) Matrigel invasion assay. Lung cancer cells were plated onto the matrigel-coated membrane in the top chamber of the transwell for 24 h. Cells invaded to the lower chambered were fixed with methanol, stained with crystal violet and counted. Data represent mean ± SD. *Significantly different from respective controls, P < 0.05.