Figure 1. (A) ETV1 reduces tolerance of Saos2 to re-expression of p53. p53-deficient Saos-2 cells harboring an expression construct for ETV1, anti-sense ETV1 (asETV1) or the corresponding empty vector (pBabePuro) were superinfected with a p53-expressing construct or the corresponding empty vector (pMX12). The cells were selected for the presence of the pMX12-encoded hygromycin resistance marker. The numbers of remaining cells were compared using methylene blue staining and extraction method. Results for each arm of the experiment were normalized for the number of cells in pBabePuro-transduced populations. (B) Tolerance to ETV1 overexpression is enhanced by interference with the function of p53. hTERT-HME1 harboring either dominant-negative p53 fragment (GSE56) or the respective empty vector control (pLXSN) were infected with an ETV1-expressing retrovirus, or the respective empty vector control (pBabePuro). The yield of puromycin-resistant cells was measured using methylene blue staining and extraction method. The results were adjusted for virus titer and are scaled relative to the numbers of cells in pBabePuro-transduced populations.