Figure 2. Effect of ETV1 and dETV1 expression on p14ARF mRNA expression. (A) Schematic representation of ETV1 deletion mutant, dETV1. The dETV1 variant lacks 131 N-terminal amino acids, which results in the loss of a transactivation domain position from amino acids 42 to 73. The DNA-binding ETS domain, as well as another putative transactivation domain spanning amino acids 437–462, remains intact at the C-terminal end. The scheme is not drawn to scale. (B) p14ARF mRNA expression in Saos-2 cells constitutively expressing ETV1 or dETV1 was measured by quantitative RT-PCR, normalized to mean GAPDH transcript levels and reported relative to that in cells harboring the respective empty vector control (pBabePuro). Results are shown as means and standard deviations of three independent replicas.