Figure 2. Biological processes affected by WA treatment in MDA-MB-231 and MCF-7 cells.

(A) The heatmap represents gene expressions for control and WA 700 nM treated MDA-MB-231 and MCF-7 cells. Only genes that have a log₂ fold change (FC)≥1 or ≤1 between control and WA-treated samples and have a p-value<0.001 are plotted. Genes that are upregulated in treated samples are plotted above the dashed line (FC≥1), those that are downregulated in treated samples are plotted below (FC≤1). Genes that belong to a specific IPA biological process are clustered and represented by a colored square next to the heatmap. Color key legend represents log₂ normalized expression values ranging from green (low expression) to red (high expression). (B) Venn diagram represents common and cell line-specific IPA biological processes with predicted activation state (Red arrow represents increase of a biological process- Activation Z-score≥2; Green arrow represents decrease of a biological process- Activation Z-score≤-2).