Table 2. The top upstream regulators of WA target genes ranked according to the Activation Z-score (≥ ±2) and p-value.
| MCF-7 | MDA-MB-231 | ||||||
| Upstream Regulator | Molecule Type | Predicted Activation State | Activation z-score | p-value of overlap | Predicted Activation State | Activation z-score | p-value of overlap |
| TP53 * | TR | Activated | 6.158 | 1.99E-49 | 1.605 | 3.65E-32 | |
| KDM5B | TR | Activated | 4.878 | 5.47E-16 | Activated | 3.337 | 1.39E-07 |
| CDKN2A | TR | Activated | 4.808 | 1.94E-19 | Activated | 3.143 | 6.81E-06 |
| NFE2L2 | TR | Activated | 3.964 | 9.27E-08 | Activated | 3.554 | 1.06E-03 |
| ATF4 | TR | Activated | 3.261 | 2.28E-10 | Activated | 3.301 | 1.35E-13 |
| EP300 | TR | Activated | 2.193 | 3.63E-05 | Activated | 2.012 | 6.12E-04 |
| PPRC1 | O | Activated | 2.000 | 2.40E-02 | Activated | 2.828 | 2.06E-02 |
| TRIB3 | K | Inhibited | -2.950 | 6.66E-10 | Inhibited | −3.554 | 3.76E-10 |
| E2F1 * | TR | Inhibited | -4.240 | 1.63E-42 | −1.776 | 5.13E-14 | |
| FOXM1 | TR | Inhibited | -4.592 | 4.39E-24 | Inhibited | −2.906 | 2.98E-02 |
| TBX2 | TR | Inhibited | -5.621 | 5.99E-32 | Inhibited | −3.446 | 2.89E-06 |
| SPDEF | TR | Activated | 4.111 | 1.17E-08 | |||
| CREM | O | Activated | 3.636 | 2.49E-07 | |||
| CREB1 | TR | Activated | 3.424 | 3.49E-13 | |||
| TRIM24 | TR | Activated | 3.207 | 1.98E-02 | |||
| SP3 | TR | Activated | 2.609 | 9.13E-03 | |||
| FOXL2 | TR | Activated | 2.512 | 7.68E-03 | |||
| ELF4 | TR | Activated | 2.449 | 6.80E-03 | |||
| SIRT1 | TR | Activated | 2.281 | 1.89E-02 | |||
| TFEB | TR | Activated | 2.254 | 1.72E-04 | |||
| ATF2 | TR | Activated | 2.236 | 5.10E-04 | |||
| HOXA5 | TR | Activated | 2.2 | 3.56E-04 | |||
| NOTCH3 | TR | Activated | 2.183 | 4.61E-02 | |||
| NFKBIA | TR | Activated | 2.134 | 5.43E-08 | |||
| EPAS1 | TR | Activated | 2.05 | 1.68E-09 | |||
| SMARCE1 | TR | Activated | 2 | 1.27E-02 | |||
| DACH1 | TR | Inhibited | −2 | 2.38E-02 | |||
| FOSL1 | TR | Inhibited | −2.201 | 1.84E-04 | |||
| MLXIPL | TR | Inhibited | −2.214 | 3.38E-02 | |||
| ERG | TR | Inhibited | −2.224 | 2.39E-04 | |||
| MAX | TR | Inhibited | −2.229 | 6.47E-05 | |||
| TCF3 | TR | Activated | 3.552 | 2.86E-11 | |||
| RBL1 | O | Activated | 3.434 | 2.29E-11 | |||
| RB1 | TR | Activated | 3.163 | 1.65E-24 | |||
| SMARCB1 | TR | Activated | 2.599 | 1.62E-14 | |||
| TOB1 | TR | Activated | 2.333 | 1.19E-06 | |||
| FOXO3 | TR | Activated | 2.225 | 3.40E-09 | |||
| NFATC2 | TR | Activated | 2.219 | 1.30E-01 | |||
| PPARG | LDNR | Activated | 2.018 | 4.45E-04 | |||
| NRIP1 | TR | Activated | 2 | 1.46E-02 | |||
| MYBL2 | TR | Inhibited | −2.149 | 1.54E-07 | |||
| NCOA3 | TR | Inhibited | −2.182 | 1.36E-02 | |||
| SMAD7 | TR | Inhibited | −2.183 | 5.08E-03 | |||
| FOXO1 | TR | Inhibited | −2.203 | 8.72E-13 | |||
| IRF7 | TR | Inhibited | −2.359 | 3.87E-03 | |||
| E2F3 | TR | Inhibited | −2.433 | 3.19E-19 | |||
| E2F2 | TR | Inhibited | −2.795 | 1.40E-16 | |||
| MYB | TR | Inhibited | −2.915 | 6.37E-05 | |||
| MYC | TR | Inhibited | −4.653 | 1.26E-18 | |||
TR- Transcription regulator; O- Other; K- Kinase; LDNR- Ligand dependent nuclear receptor. * TP53 and E2F1 transcription factors were selected for further analysis based on the lowest p-values in MDA-MB-231 despite lower than ±2 Activation Z-score.