Figure 5.

Overexpression of AsE246 results in increased numbers of nodules. A, The empty vector control hair root, inoculated with M. huakuii 7653R and grown in the absence of nitrogen. The photographs were taken 4 weeks after inoculation. B, AsE246-overexpressing hairy root inoculated with M. huakuii 7653R, showing the increase in nodule numbers. C, Close-up of boxed area in A. The nodules are indicated by white arrows. D, Close-up of boxed area in B. E, Numbers of nodules per root. The nodule number of AsE246-overexpressing roots (n = 17) is more than that of the empty vector control. F, The nitrogenase activity of AsE246 overexpressing and control nodules showed no remarkable difference. Different letters above bars indicate significant differences (P < 0.05, Student’s t test) between pairwise comparisons. Bars = 1 cm. [See online article for color version of this figure.]