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CMY FRET response to copper and mercury is impaired at high metal concentrations. Cu2+ (A) and Hg2+ (B) were titrated with CMY to monitor FRET changes. Measurement parameters were as follows: λex 440 nm/slit 5 nm, λem 460 to 560 nm/slit 5 nm. [See online article for color version of this figure.]
