Figure 4.

Pma ES4326-induced PME activity involved both the ERF1- and the MYC2-dependent branch of JA signaling. A, Pma ES4326-induced PME activity was strongly reduced in coi1. Wild-type Col-0 (Col) plants and mutants impaired in JA perception (coi1-1), JA biosynthesis (dde2-2), ET signaling (ein2-1), PAD4-dependent signaling (pad4-1), and SA biosynthesis (sid2-2) were inoculated with Pma ES4326 (OD₆₀₀ = 0.002). Leaves were harvested immediately (0 h) and after 72 h (72 h) and total PME activity was determined. B, Pma ES4326-induced PME activity was increased in the ERF1 overexpression line ERF1-1. Experiments were performed as in A. C, Pma ES4326-induced PME activity was unaltered in MYC2 single mutants jin1-1 and jin1-7 but reduced in two myc2 myc3 myc4 triple mutants. Experiments were performed as in A. Bars represent means and ses from two (A and C) or four (B) independent biological replicates with three technical replicates per sample, combined using a mixed linear model analysis. Asterisks indicate significant differences from Col-0 at each time point (q < 0.01).