Figure 4.

Relative amounts of lipids in Arabidopsis leaves from SAD6-OE and SAD6-OE-RNAi in comparison with Col-0. A, Saturated and unsaturated FAs of C16 to C18 species. B, Ratios of the total amount of each of the polar lipid classes MGDG, DGDG, PG, PC, PE, and PI. The ratios were calculated from the absolute amounts of each lipid species in the respective galactolipid and phospholipid class of three to four independent experiments. C to H, Lipid species of the polar head group classes MGDG (C), DGDG (D), PG (E), PC (F), PE (G), and PI (H). Insets show enlargements of the lipid species below 10 mol % with significant differences (P ≤ 0.05) between SAD6-OE or SAD6-OE-RNAi and the wild-type Col-0. FAs and polar lipid species were determined by LC-MS/MS, and mean values (mol % ± sd) were calculated from three to four independent experiments. Statistical analysis was performed by using an unpaired two-tailed Student’s t test: *P ≤ 0.05, **P ≤ 0.01 ***P ≤ 0.001; n.d., not determined.