The Arabidopsis fad3-2 mutant develops smaller tumors only under low RH. Tumor weights from fad3-2 (Browse et al., 1993) and fad7-1/8-1 (McConn et al., 1994) mutants and the respective wild-type Col-0 (WT) grown at RH of 45% to 50% (A) and 75% to 100% (B). Tumor weights were determined per centimeter of infected stems 3 weeks after injection of A. tumefaciens strain C58. Mean values ± se were calculated from 22 tumors of fad3-2, 18 tumors of fad7-1/8-1, and 28 tumors of Col-0 (A) and from 22 tumors of fad3-2, six tumors of fad7-1/8-1, and 24 tumors of Col-0 (B). Statistical analysis was performed using an unpaired two-tailed Student’s t test: **P ≤ 0.01.