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. 2013 Dec 18;164(2):710–720. doi: 10.1104/pp.113.228007

Figure 6.

Figure 6.

Temporal and spatial expression of BrpMIR319a2 and BrpTCP4 genes in three leaf regions. A, Northern blotting showing the expression levels of BrpMIR319a2 in the tip, middle (mid), and base of the fifth rosette leaves and the third heading leaves of the wild type (left) and 319a2-2 (right) at the heading stage. B, In situ hybridization showing the localized expression of BrpMIR319a and BrpTCP4 genes in the fifth rosette leaves and the third heading leaves of the wild type (left) and 319a2-2 (right) at the heading stage. Bars = 100 μm. C, Real-time PCR showing the relative expression of BrpTCP4-1 in the fifth rosette leaves and the third heading leaves of the wild type (left) and 319a2-2 (right) at the heading stage. ACTIN was used as the loading control. The error bars indicate three biological replicates. D, Longitudinal sections through the middle of the first head leaf at the heading stage showing H4 expression (only the top half of the section is shown). Bars = 100 μm.