Figure 2.

SICPI overexpression stimulates LeEix2-induced defense responses. A, Tobacco transiently transformed with Pro_35S:tvEix and either Pro_35S:SlCPI or Pro_35S:GFP. Induction of HR was monitored 24 and 48 h after transformation. B, Leaf discs of transiently transformed tobacco leaves with either Pro_35S:GFP (control) or Pro_35S:SlCPI were floated 24 or 48 h after transformation on a 250 mm sorbitol solution supplemented with 2.5 μg mL⁻¹ Eix. Ethylene biosynthesis was measured after 4 h. Values represent averages ± se of four different experiments. C, Electrolyte leakage was measured 24 and 48 h after transformation. Values represent average conductivity ± se of three different experiments.