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. 2013 Dec 18;164(2):819–827. doi: 10.1104/pp.113.230136

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Figure 3. — SICPI overexpression affects LeEix2 endocytosis. N. benthamiana was transiently transformed with a combination of Pro_35S:GFP-LeEix2, Pro_35S:SlCPI, and Pro_35S:FYVE-DsRed (as indicated). A, Internalization of LeEix2-GFP on FYVE-DsRed-tagged endosomes was examined 30 min (top) or 90 min (bottom) after Eix application. Arrowheads indicate the colocalization of LeEix2- and FYVE-DsRed-tagged endosomes Bars = 20 μm. B, LeEix2-GFP and FYVE-DsRed fluorescence from at least five to 10 cells of each sample was quantified using ImageJ software. All images were captured using identical conditions.