HR monitoring, ethylene production, and ion leakage in NbCPI-silenced plants. A, NbCPI-silenced and control N. benthamiana plants were transformed with a mixture of Pro35S:LeEix2 and Pro-35S:tvEix. Induction of the HR was monitored 48 and 72 h after injection. B, Leaf discs from NbCPI-silenced and control plants, transiently transformed with either Pro35S:GFP or Pro35S:LeEix2 and Pro-35S:tvEix, were floated on a 250 mm sorbitol solution supplemented with 2.5 μg mL−1 Eix at 48 and 72 h after transformation. Ethylene biosynthesis was measured after 4 h. Values represent averages ± se of five different experiments. C, Electrolyte leakage was measured after 24 and 48 h. Values represent averages ± se of three different experiments.