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Localization of LeEix2 with the endosomal marker FYVE-DsRed in leaf tissue treated with filipin. N. benthamiana leaves transiently expressing LeEix2-GFP and FYVE-DsRed were treated with Eix (2.5 μg g−1) or filipin (20 μg mL−1) and Eix (2.5 μg g−1). Leaf sections were visualized using a laser scanning confocal microscope 30 min after treatment. Arrowheads indicate the colocalization of LeEix2-GFP and FYVE-DsRed on endosomes. Bars = 10 µm.
