Figure 7.

Effect of heat stress on mimosine content and its degradation product and on the expression level of mimosinase. A, Western-blot analysis of mimosinase (45 kD) expression in leaf tissue under controlled temperature (25°C) and heat treatment (70°C). Rabbit anti-mimosinase polyclonal antibody and mouse anti-actin (plant) monoclonal antibody were used as primary antibodies for mimosinase and β-actin, respectively. B, The concentrations of mimosine and 3H4P were measured using HPLC in 0.3 g of intact leaf tissues from 8-week-old L. leucocephala. For heat treatment (t), the 8-week-old plants were incubated at 70°C for 10 min before collecting the intact leaf tissues. For each control (c) and heat treatment group, three biological replicates were used (n = 3). The bars show means of mimosine and 3H4P contents in control and treatment groups, and error bars represents sd. [See online article for color version of this figure.]